Computer-assisted transposition of conditions in high-performance thin-layer chromatography to high-performance liquid chromatography for the separation of pesticides |
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| Authors: | Qin-Sun Wang Ru-Yu Gao Heng-Yan Wang |
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| Affiliation: | (1) National Laboratory of Elemento-Organic Chemistry, Nankai University, 300070 Tianjin, People's Republic of China |
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| Abstract: | Summary A computer-assisted method is presented for mobile phase selection for the optimal separation of pesticides by HPTLC and HPLC. The system is based on a plot of solute retention value and separation criterion vs. binary mobile phase composition for graphic optimization. The result of HPTLC can be transposed to HPLC for optimal separation. The transposition equation is given. |
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| Keywords: | Thin-layer chromatography Column liquid chromatography Mobile phase optimization HPTLC transposition to HPLC |
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