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| 三嗪染料修饰曲通X-100亲和分离苹果酸脱氢酶 | |
| 引用本文： | 杨梅,唐江涛,李步海,唐尹萍.三嗪染料修饰曲通X-100亲和分离苹果酸脱氢酶[J].化学研究与应用,2011,23(8):1020-1024. |
| 作者姓名： | 杨梅 唐江涛 李步海 唐尹萍 |
| 作者单位： | 1. 广西大学化学化工学院,广西 南宁,530004 2. 中南民族大学化学与材料科学学院,分析化学国家民委重点实验室,湖北 武汉 430074 3. 湖北中医学院药学院,湖北 武汉,430065 |
| 基金项目： | 广西大学科研基金项目(XBZ090158)资助 |
| 摘 要： | 三嗪染料(Cibacmn blue F3G-A)的蒽醌部分结构类似于腺嘌呤,可以用于亲和分离以NAD+(NADP+)和FAD为辅酶的脱氢酶.三嗪染料通过亲核取代反应修饰曲通X-100,形成的三嗪染料-曲通X-100与成相聚合物吐温80、磷酸钾盐构成液-固亲和萃取体系.从猪心肌匀浆液纯化苹果酸脱氢酶的条件:三嗪染料-曲通... |
| 关 键 词： | 三嗪染料 曲通X-100 吐温80 液-固亲和萃取 苹果酸脱氢酶 |
Affinity partitioning of malate dehydrogenase with triton X-100 modified by cibacron blue F3G-A |
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| YANG Mei,TANG Jiang-tao,LI Bu-hai,TANG Yin-ping.Affinity partitioning of malate dehydrogenase with triton X-100 modified by cibacron blue F3G-A[J].Chemical Research and Application,2011,23(8):1020-1024. | |
| Authors: | YANG Mei TANG Jiang-tao LI Bu-hai TANG Yin-ping |
| Institution: | YANG Mei1,TANG Jiang-tao1,LI Bu-hai2,TANG Yin-ping3(1.School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China,2.Key Laboratory for Analytical Chemistry of the State Ethnic Affairs Commission,South-Central University for Nationalities,Wuhan 430074,3.Department of Pharmaceutical,Hubei College of Traditional Chinese Medicine,Wuhan 430065,China) |
| Abstract: | Due to the structure of anthraquinone of triazine-dye(Cibacron blue F3G-A,short as Cb)similar as adenine,Cb could be used to purify enzymes whose coenzyme was NAD+(NADP+)or FAD with affinity partitioning method.Triton X-100 modified by Cb through nucleophilic substitution reaction,Tween 80 and potassium phosphate constituted the liquid-solid affinity partitioning system.The optimal malate dehydrogenase(short as MDH)purification system from porcine cardiac muscle homogenate condition consists of triton X-100... |
| Keywords: | Ctriazine-dye Triton X-100 Tween 80 liquid-solid affinity partitioning malate dehydrogenase |
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