| 一种快速廉价的蛋白质测定方法 |
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| 引用本文： | 石磊,黄德盈,吴士筠. 一种快速廉价的蛋白质测定方法[J]. 分析测试学报, 1999, 18(3): 61-63 |
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| 作者姓名： | 石磊 黄德盈 吴士筠 |
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| 作者单位： | 武汉中南民族学院化学系,武汉,430074 |
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| 摘 要： | 蛋白质测定的Ｌｏｗｒｙ法显色反应在３０℃以下通常需３０ｍｉｎ，Ｌａｒｓｏｎ利用加入二硫苏糖醇（ＤＴＴ）使反应快速完成并提高了灵敏度，但存在ＤＴＴ价格昂贵的不足。利用连二亚硫酸钠（保险粉）代替ＤＴＴ，实现了蛋白质含量快速、灵敏、廉价的测定。
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| 关 键 词： | 连二亚硫酸钠 蛋白质 二硫苏糖醇 |
A Fast and Cheap Method for Protein Determination |
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| Shi Lei,Huang Deying,Wu Shijun. A Fast and Cheap Method for Protein Determination[J]. Journal of Instrumental Analysis, 1999, 18(3): 61-63 |
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| Authors: | Shi Lei Huang Deying Wu Shijun |
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| Abstract: | The Lowry method for protein determination usually incorporates a 30 _ minute standing period to allow full color development after the addition of Folin reagent below 30. The addition of dithiothreitol(DTT), as suggested by Larson, reduces the standing time and enhances the sensitivity as well. However, the high price of DTT is a drawback. In this paper, authors propose the substitution of DTT by a cheap reagent, sodium dithionite. Thus, the determination of proteins can be not only rapid and sensitive, but also cheap. |
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| Keywords: | Sodium dithionite Protein Dithiothreitol |
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