四妙勇安汤抑制动脉粥样硬化易损斑块炎症反应机制

 通过利用复合因素制备兔动脉粥样硬化（AS）易损斑块模型，研究四妙勇安汤对AS易损斑块的影响及作用机制。随机将40只日本大耳白兔分为正常组10只，实验组30只。正常组予普通饲料，实验组利用复合因素制备兔主AS易损斑块模型，8周时实验组随机分为模型组、辛伐组、四妙组，继续予以高脂饮食；各给药组开始给药至24周取材，观察主动脉的病理形态，检测血清MCP-1、ICAM-1、CRP含量和斑块内HSP60、TNF-α、NF-κB表达。结果显示，模型组内膜明显增厚、纤维帽较薄，斑块内有大量脂质沉积和炎症细胞浸润，血清MCP-1、ICAM-1、CRP随时间延长呈上升趋势，主动脉斑块TNF-α、HSP60、NF-κB表达增加。在给药后各时间点四妙组血清MCP-1、ICAM-1水平均低于模型组（P<0.05或P<0.01），对CRP水平影响不明显。斑块内TNF-α、HSP60、NF-κB阳性面积百分比均低于模型组（P<0.01），ICAM-1 MRNA表达低于模型组（P<0.01）。由此得出，四妙勇安汤能够稳定AS易损斑块，其作用可能是通过抑制炎症反应实现的。

Effect of Simiaoyong'an Decoction on Inflammation Reaction in Rabbit's Atherosclerosis Vulnerable Plaque

The purpose of this study is to observe the effect of Simiaoyong'an Decoction (SYD) on Atherosclerosis (AS) vulnerable plaque. 40 Japanese white rabbits were randomly assigned to two groups: 10 in the normal control group, and 30 in the experimental group to be established as the AS model by high fatty diet feeding, combined with immune injury at the 2nd week and femoral arterial balloon tearing at the 4th week. At the 8th week, 30 rabbits in the experimental group were randomly assigned to four groups: each 10 in the model group, the SYD group and the simvastatin group. The medication of SYD and simvastatin were given in the whole 16-week course. Levels of monocyte chemotactic protein-1 (MCP-1), and intercellular adhesion molecule (ICAM-1) and CRP were measured at the 8th, 16th and 24th weekend in experiment. The tumor necrosis factor alpha (TNF-α) and the nuclear factor-kappa B (NF-κB), HSP60 expression in aortic wall were measured at the end of experiment. The results show that compared with the model group, the levels of MCP-1, ICAM-1 are lower in the two treated groups at all time points after treatment (P<0.01 or P<0.05). TNF-α, NF-κB and HSP60 expression in aortic wall are lower after treatment (P<0.01 or P<0.05). In overall, it is demonstrated that SYD could stabilize atherosclerosis vulnerable plaque by suppressing the inflammation reaction.