亲和色谱仿生配基的筛选和设计

利用选择性好、稳定性高、价格低廉的仿生配基作为亲和色谱的功能基团，实现对目的蛋白质的亲和分离，可以弥补常规的单克隆抗体和其他天然蛋白质配基在价格、稳定性方面的不足，从而拓展亲和色谱技术的应用范围。本文综述了亲和色谱仿生配基在筛选和设计方面的研究进展，重点介绍了组合方法和基于结构的仿生配基的设计及其应用。

Selection and Design of Biomimetic Ligands for Affinity Chromatography

Affinity chromatography is regarded as the most sophisticated and powerful purification technique. However, the commonly used biological ligands tend to be expensive, chemically and biologically labile, such a problem limites its application in large-scale bioprocessing. The biomimetic ligands selected and designed by various methods would be instead of biospecific ligands in the purification of high value biopharmaceutical proteins since their advantages in price and stability. In this paper, the recent investigations related to biomimetic ligands and the rapidly screening and synthesizing methods were reviewed and discussed. Emphasis is placed on combinatorial approaches and structure-based rational ligand design.