Selective enhancement of nucleases by polyvalent DNA-functionalized gold nanoparticles |
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Authors: | Prigodich Andrew E Alhasan Ali H Mirkin Chad A |
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Affiliation: | Department of Chemistry and International Institute of Nanotechnology, Northwestern University, Evanston, Illinois 60208, United States. |
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Abstract: | We demonstrate that polyvalent DNA-functionalized gold nanoparticles (DNA-Au NPs) selectively enhance ribonuclease H (RNase H) activity while inhibiting most biologically relevant nucleases. This combination of properties is particularly interesting in the context of gene regulation, since high RNase H activity results in rapid mRNA degradation and general nuclease inhibition results in high biological stability. We have investigated the mechanism of selective RNase H activation and found that the high DNA density of DNA-Au NPs is responsible for this unusual behavior. This work adds to our understanding of polyvalent DNA-Au NPs as gene regulation agents and suggests a new model for selectively controlling protein-nanoparticle interactions. |
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