Microfluidic Synthesis of Rigid Nanovesicles for Hydrophilic Reagents Delivery |
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| Authors: | Lu Zhang Qiang Feng Jiuling Wang Prof. Dr. Jiashu Sun Prof. Dr. Xinghua Shi Prof. Dr. Xingyu Jiang |
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| Affiliation: | 1. Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, No.11 ZhongGuanCun BeiYiTiao, Beijing, 100190 (P. R. China);2. State Key Laboratory of Nonlinear Mechanics, Institute of Mechanics, Chinese Academy of Sciences, No.15 Beisihuanxi Road, Beijing, 100190 (P. R. China) |
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| Abstract: | We present a hollow‐structured rigid nanovesicle (RNV) fabricated by a multi‐stage microfluidic chip in one step, to effectively entrap various hydrophilic reagents inside, without complicated synthesis, extensive use of emulsifiers and stabilizers, and laborious purification procedures. The RNV contains a hollow water core, a rigid poly (lactic‐co‐glycolic acid) (PLGA) shell, and an outermost lipid layer. The formation mechanism of the RNV is investigated by dissipative particle dynamics (DPD) simulations. The entrapment efficiency of hydrophilic reagents such as calcein, rhodamine B and siRNA inside the hollow water core of RNV is ≈90 %. In comparison with the combination of free Dox and siRNA, RNV that co‐encapsulate siRNA and doxorubicin (Dox) reveals a significantly enhanced anti‐tumor effect for a multi‐drug resistant tumor model. |
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| Keywords: | drug delivery hydrophilic reagents microfluidics nanoparticles vesicles |
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