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Amphipathic DNA Origami Nanoparticles to Scaffold and Deform Lipid Membrane Vesicles |
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| Authors: | Dr Aleksander Czogalla Dominik J Kauert Dr Henri G Franquelim Dr Veselina Uzunova Dr Yixin Zhang Prof Ralf Seidel Prof Petra Schwille |
| Institution: | 1. Biotechnology Center of the TU Dresden, Tatzberg 47/51, 01307 Dresden (Germany);2. Department of Cytobiochemistry, Faculty of Biotechnology, University of Wroc?aw ul. F. Joliot‐Curie 14a, 50383 Wroc?aw (Poland);3. Institute for Molecular Cell Biology, University of Münster, Schlossplatz 5, 48149 Münster (Germany);4. Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried (Germany) http://www.biochem.mpg.de/en/rd/schwille/;5. B CUBE—Center for Molecular Bioengineering, Dresden, University of Technology, Arnoldstra?e 18, 01307 Dresden (Germany) |
| Abstract: | We report a synthetic biology‐inspired approach for the engineering of amphipathic DNA origami structures as membrane‐scaffolding tools. The structures have a flat membrane‐binding interface decorated with cholesterol‐derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free‐standing lipid membranes, mimicking the biological activity of coat‐forming proteins, for example, from the I‐/F‐BAR family. |
| Keywords: | diffusion lipid membranes membrane deformation oligomerization origami DNA structures |