Development of Imidazoline‐2‐Thiones Based Two‐Photon Fluorescence Probes for Imaging Hypochlorite Generation in a Co‐Culture System |
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| Authors: | Dr. Qingling Xu Cheol Ho Heo Gyoungmi Kim Hyo Won Lee Prof. Hwan Myung Kim Prof. Juyoung Yoon |
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| Affiliation: | 1. Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120‐750 (Korea);2. Department of Energy Systems Research, Ajou University, Suwon, Gyeonggi‐do 443‐749 (Korea) |
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| Abstract: | We designed and prepared the imidazoline‐2‐thione containing OCl? probes, PIS and NIS , which operate through specific reactions with OCl? that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl? generation in macrophages in a co‐culture system. We have also employed two‐photon microscopy and PIS to image OCl? in live cells and tissues, indicating that this probe could have wide biological applications. |
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| Keywords: | co‐culture systems fluorescence probes hypochlorite generation two‐photon microscopy |
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