蛋白吸附的光谱分析方法及其对生物材料的合成指导

综述了生物医用材料和血液接触后,不同血浆蛋白组分在材料表面的竞争吸附行为;材料表面特性对血浆蛋白吸附有重要影响,具有表面自由能小、亲水性强、带有负电荷和存在有微相分离结构的材料均能降低血浆纤维蛋白原的吸附,表现出良好的抗凝血性能;总结了红外、圆二色谱、核磁、荧光等光谱学手段在蛋白吸附表征方面的研究进展,XPS、Raman、AFM等现代仪器也崭露头角,其中,石英晶体微天平对吸附机理的研究显得更直观;探讨了指导抗凝血生物医用材料的设计思路,指出蛋白吸附的深入研究有助于提高生物材料设计的水平。

Spectrum analysis methods of protein adsorption and design of biomedical materials

Protein absorption happens firstly when biological materials contact environment of organisms. The competitive adsorption behavior of different protein and the impact of biomaterial surfaces characteristics on protein adsorption are summarized. Materials with small surface free energy, high hydrophility, and a negative charge, or with the presence of micro-phase separation structure are able to reduce fibrinogen adsorption, showing good anti-clotting properties. FTIR, CD, NMR and FL are applied in protein adsorption analysis. XPS, Raman, AFM and other modern instruments have also emerged in this area. QCM appears to be more intuitive in the study of protein adsorption mechanism. The development of study on protein adsorption would guide the design of biomedical materials.