| PROPERTIES OF CHICKEN LIVER PHOSPHOFRUCTOKINASE-2 |
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| 引用本文： | 李林,潘力,许根俊. PROPERTIES OF CHICKEN LIVER PHOSPHOFRUCTOKINASE-2[J]. 中国科学B辑(英文版), 1991, 0(8) |
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| 作者姓名： | 李林 潘力 许根俊 |
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| 作者单位： | Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai Institute of Biochemistry，Academia Sinica |
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| 基金项目： | Supported by the National Natural Science Foundation of China |
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| 摘 要： | Phosphofructokinase-2 was purified to homogeneity from chicken livers by homogeniza-tion, polyethylene glycol fractionation and column chromatography on DEAE-Sephadex A-50 and Blue-Sepharose 4B. Some properties of the enzyme were as follows: (i) The saturation curve of the enzyme for fructose 6-phosphate showed hyperbolic and the Km of fructose 6-phosphate was affected by inorganic phosphate while Vmax was not; (ii) the binding of ATP to the enzyme was of negative cooperativity with a Hill coefficient of 0.56; (iii) the activity of the enzyme was completely lost in the presence of EDTA. The enzyme was activated by Mg2+ at low concentrations, but inhibited by Mg2+ at high concentrations; (iv) the enzyme was stable below 30℃ and easily lost its activity when the temperature was above 40℃; (v) the activity of the enzyme was stable at the range of pH 7-9, increased at pH 9.0-9.5 and decreased when pH was over 9.5; (vi) the enzyme was sensitive to trypsin and ATP protected the enzyme against the proteolysi
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PROPERTIES OF CHICKEN LIVER PHOSPHOFRUCTOKINASE-2 |
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| LI LIN PAN LI AND XU GEN-JUN. PROPERTIES OF CHICKEN LIVER PHOSPHOFRUCTOKINASE-2[J]. Science in China(Chemistry), 1991, 0(8) |
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| Authors: | LI LIN PAN LI AND XU GEN-JUN |
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| Abstract: | |
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| Keywords: | phosphofruetokinase-2 kinetic properties purification. |
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