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improvement of the Specificity of Surface Plasmon Resonance with BSA-modified Chip
作者姓名:Li  Hua  CHEN
作者单位:Li Hua CHEN? College of chemistry and molecular engineer,Qingdao University of Science and Technology,Qingdao 266042
基金项目:教育部留学回国人员科研启动基金
摘    要:Recently, surface plasmon resonance (SPR) become more and more popular without the need of the label technology1-3. However, sometimes, a number of experimental artifacts complicate the final biosensor analysis4-7. The utilization of a reference surface c…

关 键 词:牛血清白蛋白  BSA修饰芯片  谷胱甘肽S转移酶  表面等离子体子谐振  SPR  特异性  改良
收稿时间:2006-09-01

Improvement of the Specificity of Surface Plasmon Resonance with BSA-modified Chip
Li Hua CHEN.improvement of the Specificity of Surface Plasmon Resonance with BSA-modified Chip[J].Chinese Chemical Letters,2006,17(12):1619-1622.
Authors:Li Hua CHEN
Abstract:A chip was modified with bovine serum albumin (BSA), then interaction between glutathione (GSH) immobilized on the top of BSA and glutathione-S-transferase (GST) was examined, using surface plasmon resonance (SPR). The SPR results showed that BSA-modified chip was effective not only in binding the target proteins but also in suppressing the nonspecific binding (NSB) of proteins.
Keywords:Surface plasmon resonance (SPR)  bovine serum albumin (BSA)  glutathione (GSH)  glutathione-S-transferase (GST)  
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