| STUDIES ON HETEROLOGOUS EXPRESSION OF Rhizobium meliloti nifA GENE AND OXYGEN SENSITIVITY OF ITS PRODUCT |
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| 引用本文： | 王水平,朱家壁,俞冠翘,吴一飞,沈善炯. STUDIES ON HETEROLOGOUS EXPRESSION OF Rhizobium meliloti nifA GENE AND OXYGEN SENSITIVITY OF ITS PRODUCT[J]. 中国科学B辑(英文版), 1991, 0(1) |
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| 作者姓名： | 王水平 朱家壁 俞冠翘 吴一飞 沈善炯 |
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| 作者单位： | Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，Laboratory of Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC |
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| 基金项目： | Project supported by the National Natural Science Foundation of China and partially by the Biotechnology Career Fellowship of Rockefeller Foundation (for ZHU Jia-bi's work). |
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| 摘 要： | When the R. meliloti (Rm) nifA'-lacZ fusion-carrying plasmids were introduced intothe strains of Agrobacterium tumefaciens, R. trifolii and R. astragalus, β-galactosidaseactivity was demonstrated. However, activity was not induced by microaerobiosis. Further-more, R. meliloti nifA'-lacZ fusion was also not expressed in the nodule bacteroids ofR. trifolii and R. astragalus. We speculate that some factor(s) important for the inductionof Rm nifA presumed to be the fixLJ regulatory system would not be operative in thesebacteria. Experiments using R. meliloti nifH'-lacZ/ K. Pneumoniae nifH'-lacZ fusion and theconstitutive Rm nifA system to test the nifA-dependent expression of nifH'-lacZ underaerobic and microaerobic conditions in E. coli were performed. The inhibition of the RmnifA activation of nifH'-lacZ expression in the bacteria grown in the aerobic conditionwas shown. Assays on the Rm nifA-m RNA produced by the constitutive Rm nifA in E.coli under aerobic and microaerobic conditions with the cloned ni
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STUDIES ON HETEROLOGOUS EXPRESSION OF Rhizobium meliloti nifA GENE AND OXYGEN SENSITIVITY OF ITS PRODUCT |
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| WANG SHUI-FING,ZHU JIA-BI,YU GUAN-QIAO,WU YI-FEI,SHEN SHAN-JIONG Laboratory of Molecular Genetics. STUDIES ON HETEROLOGOUS EXPRESSION OF Rhizobium meliloti nifA GENE AND OXYGEN SENSITIVITY OF ITS PRODUCT[J]. Science in China(Chemistry), 1991, 0(1) |
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| Authors: | WANG SHUI-FING ZHU JIA-BI YU GUAN-QIAO WU YI-FEI SHEN SHAN-JIONG Laboratory of Molecular Genetics |
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| Affiliation: | WANG SHUI-FING,ZHU JIA-BI,YU GUAN-QIAO,WU YI-FEI,SHEN SHAN-JIONG Laboratory of Molecular Genetics,Shanghai Institute of Plant Physiology,Academia Sinica,Shanghai 200032,PRC |
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| Abstract: | |
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| Keywords: | Rhizobium meliloti nifA gene gene expression oxygen sensitivity |
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