| Assay of Histamine in Single Mast Cells by Capillary Zone Electrophoresis with Electrochemical Detection |
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| 作者姓名： | Qian Feng WENG Fang Quan XIA Wen Rui JIN* School of Chemistry and Chemical Engineering Shandong University Jinan |
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| 作者单位： | Qian Feng WENG，Fang Quan XIA，Wen Rui JIN* School of Chemistry and Chemical Engineering，Shandong University，Jinan 250100 |
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| 摘 要： | Histamine is one of the chemical mediators in connection with allergies. Only Pihel et al.1 reported a method for detection of histamine in isolated mast cells by high- performance liquid chromatography with electrochemical detection. In this method, single cells were removed and transferred to 300-nL microvials. A part of the supernatant from individual vials was injected into the chromatography column. The analysis of histamine in individual rat peritoneal mast cells using CZE with amp…
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Assay of Histamine in Single Mast Cells by Capillary Zone Electrophoresis with Electrochemical Detection |
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| Qian Feng WENG,Fang Quan XIA,Wen Rui JIN* School of Chemistry and Chemical Engineering,Shandong University,Jinan .Assay of Histamine in Single Mast Cells by Capillary Zone Electrophoresis with Electrochemical Detection[J].Chinese Chemical Letters,2002,13(10). |
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| Authors: | Qian Feng WENG Fang Quan XIA Wen Rui JIN |
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| Institution: | School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 |
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| Abstract: | Capillary zone electrophoresis was employed for the analysis of histamine in single rat peritoneal mast cells using an amperometric detector. In this method, individual mast cells and then 0.02 mol/L NaOH as a lysing solution are injected into the front end of the separation capillary. A cell injector was constructed for easy injection of single cells. Histamine in single mast cells has been identified and quantified. |
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| Keywords: | Capillary electrophoresis electrochemical detection histamine mast cell |
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