肺泡巨噬细胞吞噬粉尘时的化学发光现象

肺泡巨噬细胞(Alveolar macrophages AMs)在吞噬异物的过程中会产生吞噬相伴随韵化学发光现象,但是非常微弱,加入鲁米诺(Luminol)可增强发光,用液体闪烁计数器可测到这种发光。本文用化学发光法(Chemiluminescence CL)试验了AMs浓度、鲁米诺浓度以及酵母聚糖(Zymosan)浓度与化学发光强度的关系,检测了接触不同粉尘的AMs的CL动态变化,用CL强度来判断AMs的CL吞噬功能。试验表明,CL强度随染尘时间的延长面减弱,它可反映粉尘毒性大小和对机体的早期损伤,这对探讨尘肺的发病机理有重要意义。

CHEMILUMINESCENCE PHENOMENON OF ALVEOLAR MACROPHAGES EXPOSING TO PARTICLES

Chemiluminesctnce from alveolar macrophages is caused by phagocytosis. It had been enhanced by adding luminol and measured on a liquid scintillation counter. The quartz and CaCO3 particles was injected into SD rats by trachea respectively. Animals were anesthetized with sodium pentobarbitol and exsar-guinated by cutting the abdominal arota. Alveolar macrophages were harvested by pulmonary lavage in situ with medium. The results are showed as follows.The relationship of the concentration of alveolar macrophages, luminol, and zymosan with the intensity of CL was investigated. The best assay system is that a) alveolar macrophagts arc 2×106ce.ll/ml.b) luminol is 2×10-4M, and c) zymosan is 1×108/ml. The dynamic change of CL from alveolar macrophages in rats after exposing to particles was monitored and showed that alveolar macrophagts of the rats exposed to quartz and CaCO3 varied distinctly with one of normal rats on phagocyte-luminescence. It can respond to the toxicity of particles. The results, are showed that the intensity of CL was descended with the time of exposure to particles. The intensity of CL was used to determine phagocytic function of alveolar macrophages and early damage in organism. It is significant to research pathogeny of pncumoconisis.