Stabilization of the structure and unctions of a photosystem i submembrane fraction by immobilization in an albumin-glutaraldehyde matrix |
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Authors: | Danielle Bonenfant Robert Carpenter |
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Institution: | (1) Center de recherche en photobiophysique, Université du Québec a, Trois-Rivières, C.P. 500, G9A 5H7 Trois-Rivières, Québec, Canada |
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Abstract: | The effect of immobilization in an albumin-glutaraldehyde crosslinked matrix on the structure and activity of a photosystem
I submembrane fraction has been studied. The photosynthetic activity recovered after immobilization was between 35 and 45%
of the oxygen-uptake rates of the native material. Resulting oxygen uptake activities found in immobilized photosystem I preparations
with methylviologen as acceptor were as high as 270 μmol O2 (mg Chl h)-1, An enhancement of photosystem I electron transfer, which is produced by incubation of thylakoid membranes at temperatures
above 30 °C, was detected in native submembrane fractions, but not in the immobilized preparations. It is suggested that the
increased activity at high temperature results from conformational modifications not allowed in the immobilization matrix.
The insensitivity of immobilized photosystem I particles to prolonged storage at 4°C and to strong light exposure, as well
as their high electron-transfer rates, demonstrates that the immobilization procedure used can be successfully applied to
submembrane fractions. |
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Keywords: | Photosystem I submembrane fraction immobilization electron transfer biotechnology |
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