Rapid Fluorometric Screening of Antibiotics in Seafood |
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Authors: | Yang Liu Bengt Danielsson |
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Institution: | (1) Pure and Applied Biochemistry, Lund University, P.O. Box 124, SE-22100 Lund, Sweden;(2) Wuhan Institute of Virology, Chinese Academy of Sciences, 430071 Wuhan, Hubei, P.R. China |
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Abstract: | Simple and rapid fluorometric screening methods have been developed based on the competitive binding between the target and
an intercalating fluorophore dye to double-stranded-DNA (dsDNA). In this study, the long-wavelength fluorescente dye TOTO-3
was employed as the indicator. Compounds that interact with dsDNA will affect the binding of TOTO-3 to the nucleic acid thereby
changing the fluorescence intensity. The analyte concentration is indirectly determined by the decrease in fluorescence intensity.
A fiber optic fluorescence screening system was developed for rapid and convenient sample processing. Lambda DNA (48.5 kb)
was chosen as a suitable sensing nucleic acid material. Detection of sulfathiazole and chloramphenicol in shrimps using this
method was studied in the range of 0.5–25 ng mL−1 of sulfathiazole and of 1–50 ng mL−1 of chloramphenicol. Detection limits of 0.5 ng mL−1 of sulfathiazole and 1 ng mL−1 of chloramphenicol were achieved. This approach is useful as a routine test in the monitoring of antibiotics in the environment
or aquaculture products. The easy operation and the rapid and sensitive detection make this a potential high-throughput screening
method. |
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Keywords: | : Chloramphenicol seafood contamination sulfathiazole DNA-based drug screening fluorometric screening |
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