| 人体胆结石难溶物的扫描电镜观察和红外光谱研究 |
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| 引用本文： | 刘刚,邢达,杨海珉,吴杰.人体胆结石难溶物的扫描电镜观察和红外光谱研究[J].光谱学与光谱分析,2002,22(4):591-595. |
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| 作者姓名： | 刘刚 邢达 杨海珉 吴杰 |
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| 作者单位： | 1. 华南师范大学激光生命科学研究所,广东,广州,510631
2. 昆明医学院基础部,云南,昆明,650031 |
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| 基金项目： | 国家杰出青年基金 (No 6972 50 0 9)，广东省自然科学基金 (No 0 0 0 679)资助 |
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| 摘 要： | 用傅里叶变换红外光谱仪和扫描电镜研究了胆色素型和混合型胆结石经氯仿、乙醇、乙醚、盐酸等溶剂溶解剩余的主要组分和微观结构。结果表明，胆红素盐、蛋白质、脂肪酸盐、碳酸钙等为难溶物；棕色色素胆石中的蛋白质的二级结构似a-螺旋为主。扫描电镜观察显示，在色素型和混合型胆石难溶物中都出现了可能为蛋白质的薄膜带状物，讨论了难溶物与结石形成的关系。
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| 关 键 词： | 人体 胆结石 难溶物 扫描电镜 红外光谱 |
| 文章编号： | 1000-0593(2002)04-0591-05 |
| 修稿时间： | 2001年5月16日 |
Study of the Insoluble Remainder of Human Gallstones by Scanning Electron Microscope and Fourier Transform Infrared Spectrum |
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| Gang Liu,Da Xing,Hai-min Yang,Jie Wu.Study of the Insoluble Remainder of Human Gallstones by Scanning Electron Microscope and Fourier Transform Infrared Spectrum[J].Spectroscopy and Spectral Analysis,2002,22(4):591-595. |
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| Authors: | Gang Liu Da Xing Hai-min Yang Jie Wu |
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| Institution: | Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China. |
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| Abstract: | The insoluble remainder of brown pigment and mixed gallstones which were dissolved with chloroform, ethanol, ether, and hydrochloric acid were studied by Fourier transform infrared (FT-IR) spectrum and scanning electron microscope. It was found that bilirubinate salts, proteins, fatty salts, phosphate and calcium carbonate are the main components in the insoluble remainder of gallstones. The secondary structure of proteins in brown pigment gallstones may be predominated by alpha-helix. The microstructure of proteins in brown pigment stones is membranous, phosphate and bilirubinate salts were trapped within the membranes. The relationship between the insoluble components and formation of gallstones was discussed. |
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| Keywords: | Gallstone Insoluble remainder Infrared spectrum Scanning electron microscope |
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