| 乙基紫标记分光光度法测定脱氧核糖核酸 |
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| 引用本文： | 李天剑 沈含熙. 乙基紫标记分光光度法测定脱氧核糖核酸[J]. 分析化学, 1998, 26(11): 1372-1375 |
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| 作者姓名： | 李天剑 沈含熙 |
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| 作者单位： | 南开大学化学系!天津 300071 |
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| 基金项目： | 国家自然科学基金!（29675009） |
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| 摘 要： | 研究了阳离子染料乙基紫与脱氧核糖核酸的结合反应，在ｐｈ＝６．４－７．４的条件下，乙基紫在５９５ｎｍ处有最大吸收峰，当加入ＤＮＡ后，乙基紫的吸收峰显著下降，而在５０７ｎｍ处出现新的吸收峰。以乙基紫为标记物，根据其５９５ｎｍ吸收峰下降的程度，可用于ＤＮＡ的定量测定。
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| 关 键 词： | 乙基紫 分光光度法 DNA 结合反应 分析 |
Spectrophotometric Determination of Deoxyribonucleic Acid Labeling With Ethyl Violet |
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| Li Tianjian,Shen Hanxi,Luo Yunjing. Spectrophotometric Determination of Deoxyribonucleic Acid Labeling With Ethyl Violet[J]. Chinese Journal of Analytical Chemistry, 1998, 26(11): 1372-1375 |
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| Authors: | Li Tianjian Shen Hanxi Luo Yunjing |
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| Abstract: | The binding reaction of DNA (calf thymus deoxyribonucleic acid) with dye ethyl violet has been studied. In the medium of pH range 6 .4 - 7. 4, ethyl violet showed a decrease in absorption at the wavelength of its maximal absorption peak (595 um) on binding to DNA, and at the same time a new peak of the binding product appeared at 507 um. A novel method for the determination of DNA at 595 um using ethyl violet as a labeling agent was developed. The method is of high sensitivity as well as selectivity, and has been used to determine DNA in synthetic Samples with satisfactory results. |
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| Keywords: | Ethyl violet deoxyribonucleic acid spectrophotometry |
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