| 2,9-双甲基苯并噻唑乙烯基邻菲罗琳与DNA 的相互作用及应用研究 |
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| 引用本文： | 常昂,刘祥军,吴尚荣,柳影,王林林,张楠,李松青,上官棣华.2,9-双甲基苯并噻唑乙烯基邻菲罗琳与DNA 的相互作用及应用研究[J].化学通报,2015,78(12):1101-1101. |
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| 作者姓名： | 常昂 刘祥军 吴尚荣 柳影 王林林 张楠 李松青 上官棣华 |
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| 作者单位： | 中科院化学研究所,中科院化学研究所,中科院化学研究所,中科院化学研究所,中科院化学研究所,中科院化学研究所,湘潭大学化学学院,中国科学院化学研究所 |
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| 基金项目： | 国家“九七三”计划项目( 批准号: 2011CB935800,2013CB933700) 和国家自然科学基金(批准号：21275149,21375135,21321003) 资助。 |
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| 摘 要： | 设计合成了基于2，9-双苯并噻唑乙烯基取代的邻菲罗琳荧光分子—PMBT。通过吸收光谱、荧光光谱、圆二色光谱探讨了PMBT与不同DNA的相互作用。发现PMBT与DNA存在两种不同的相互作用模式。 由于PMBT分子中带有两个正电荷，当PMBT与DNA的浓度比值较高（大于4）时，PMBT以DNA为模板按一定方向在DNA上聚集；当PMBT与DNA的浓度比小于2时，PMBT通过嵌插或末端堆积的方式分别与单/双链DNA和G-四链体DNA结合。PMBT与DNA结合导致其荧光淬灭，利用该特性将PMBT与DNA结合构建荧光增强型检测平台，可用于DNA酶活性以及DNA降解的动力学研究。
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| 关 键 词： | 邻菲罗琳衍生物 DNA 相互作用 荧光探针 |
| 收稿时间： | 2015/6/23 0:00:00 |
| 修稿时间： | 2015/7/28 0:00:00 |
Interaction of a Phenanthroline Derivative with DNA and its Application |
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| CHANG Ang,LIU Xiangjun,WU Shangrong,LIU Ying,WANG Linlin,ZHANG Nan,LI Songqing and SHANGGUAN Dihua.Interaction of a Phenanthroline Derivative with DNA and its Application[J].Chemistry,2015,78(12):1101-1101. |
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| Authors: | CHANG Ang LIU Xiangjun WU Shangrong LIU Ying WANG Linlin ZHANG Nan LI Songqing and SHANGGUAN Dihua |
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| Institution: | Beijing National Laboratory for Molecular Sciences,Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences,,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Analytical Chemistry for Living Biosystems,Institute of Chemistry,Chinese Academy of Sciences |
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| Abstract: | A phenanthroline derivative, 2,9-bis(2-(3-methylbenzod]thiazol-2-yl)vinyl)-1,10- phenanthroline (PMBT), was designed and synthesized. The interaction of PMBT with different DNA forms was investigated by UV-Vis absorption spectrum, fluorescence spectrum and circular dichroism spectrum. PMBT possesses a large conjugated planar structure, and binds to DNA with different modes dependent on the molecular ratio of PMBT and DNA. At high molecular ratio (>4), PMBT binds to DNA through DNA-templated aggregation because of the two positive charges on each of PMBT. At low molecular ratio (<2), PMBT binds to DNA through intercalation between the DNA bases or end-stacking on G-quadruplexes. PMBT has good solubility in aqueous solution, and exhibits strong fluorescence. The fluorescence is quenched when bound to DNA. Thus PMBT was used as a fluorescent probe for investigation of DNA degradation in serum and the activity of nuclease. |
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| Keywords: | 1 10-phenanthroline DNA interaction Fluorescent probe |
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