Ultra-trace determination of S-nitrosothiols in blood samples by spectrofluorimetry with 8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene |
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Authors: | Huang Ke-Jing Xu Chun-Xuan Xie Wan-Zhen Zhang Hua-Shan Wang Hong |
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Affiliation: | Department of Chemistry, Wuhan University, Wuhan 430072, PR China. |
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Abstract: | Increasing evidence suggests that S-nitrosothiols (RSNO) may represent naturally occurring nitric oxide (NO) surrogates and function as intermediates in NO metabolism. In this work, a simple, sensitive, and selective micromethod is developed and validated for quantification of RSNO. A fluorescent probe 8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacence (DABODIPY) is firstly used to label RSNO. The derivatization reaction is performed in aqueous medium at 30 degrees C for 15min in the presence of 6x10(-5)molL(-1)Hg2+ and the derivative is detected by fluorescence at lambda(ex)/lambda(em)=500/510nm. A linear function of concentration in the range of (2.0-600.0)x10(-8)molL(-1) is observed with a correlation coefficient of 0.9992 and detection limit of 1.2x10(-9)molL(-1) (S/N=3). This technique has been successfully applied to quantify RSNO in some human blood samples including healthy persons and patients suffering from cardiovascular diseases. |
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