Methods of assessment of atropine and scopolamine levels in transdermal permeation

Three methods of atropine and scopolamine determination in transdermal permeation have been developed. Radiometric determination seems to be an excellent, quick and extremely sensitive method, which ought to be preferred when working with biological systems. The radioreceptor analysis is of group specificity. It can be used to advantage in monitoring atropine plasma concentrations exceeding 1.5 ng cm^–3 and scopolamine concentrations above 50 pg cm^–3. GC/MS provides adequate specificity and sensitivity for the determination of therapeutic levels of atropine and scopolamine in blood. The sensitivity of this method is approximately 1–2 ng cm^–3.