Rapid identification of bacteria with a disposable colorimetric sensing array |
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Authors: | Carey James R Suslick Kenneth S Hulkower Keren I Imlay James A Imlay Karin R C Ingison Crystal K Ponder Jennifer B Sen Avijit Wittrig Aaron E |
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Affiliation: | Department of Applied Chemistry, National University of Kaohsiung, 700 Kaohsiung University Road, Kaosiung 811, Taiwan. jcarey@nuk.edu.tw |
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Abstract: | Rapid identification of both species and even specific strains of human pathogenic bacteria grown on standard agar has been achieved from the volatiles they produce using a disposable colorimetric sensor array in a Petri dish imaged with an inexpensive scanner. All 10 strains of bacteria tested, including Enterococcus faecalis and Staphylococcus aureus and their antibiotic-resistant forms, were identified with 98.8% accuracy within 10 h, a clinically important time frame. Furthermore, the colorimetric sensor arrays also proved useful as a simple research tool for the study of bacterial metabolism and as an easy method for the optimization of bacterial production of fine chemicals or other fermentation processes. |
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