USE OF SYNTHETIC POLYNUCLEOTIDES TO CHARACTERISE AN ANTISERUM MADE AGAINST UV-IRRADIATED DNA |
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| Authors: | David L Mitchell Judith M Clarkson |
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| Institution: | The University of Texas System Cancer Center, Science Park, Research Division, P.O. Box 389, Smithville, TX 78957, USA |
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| Abstract: | UV—irradiated single-stranded synthetic homopolymers were used to characterise an antiserum made against UV-irradiated DNA. Using induction data obtained by reversed-phase high performance liquid chromatography, the relative antigenicity of the polymers could be converted to relative binding affinity for each DNA photoproduct. Since no Pyr(6–4)Pyo adducts could be detected at 320 nm under these conditions, the affinity of the antiserum for this lesion could be compared with that for cyclobutane dimers. The relative binding capacity of Thy(6–4)Thy: Thy(5–6)Thy: Cyt(6–4)Cyt: Cyt(5–6)Cyt = 166: 1: 0.25: 0.062. |
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