Determination of active components of Ginkgo biloba in human urine by capillary high-performance liquid chromatography/mass spectrometry with on-line column-switching purification |
| |
Authors: | Ding Shujing Dudley Ed Chen Lijuan Plummer Sue Tang Jiandong Newton Russell P Brenton A Gareth |
| |
Affiliation: | Mass Spectrometry Research Unit, Grove Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK. |
| |
Abstract: | Ginkgo biloba is one of the most popular herbal nutritional supplements, with terpene lactones and flavonoids being the two major active components. An on-line purification high-performance liquid chromatography/mass spectrometry (HPLC/MS) method was successfully developed for the quantitative determination of flavonoids and terpene lactones excreted in human urine after ingesting the herbal supplement. Satisfactory separation was obtained using a C18 capillary column made in-house with sample clean-up and pre-concentration achieved using a C18 pre-column with column switching. High selectivity and limits of detection of 1-18 ng/mL were achieved using a selected ion monitoring (SIM) scan in negative ion mode; the on-line solid-phase extraction (SPE) recovery of the active components in Ginkgo biloba determined in this study was greater than 75%. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|