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二亚油酰磷脂酰胆碱分析
引用本文:曹栋. 二亚油酰磷脂酰胆碱分析[J]. 分析试验室, 2007, 26(3): 11-14
作者姓名:曹栋
作者单位:江南大学食品学院,无锡,214036
摘    要:通过HPLC/HPLC-MS研究了二亚油酰磷脂酰胆碱的测定方法,磷脂样品首先经过HPLC分离得到磷脂酰胆碱,该磷脂酰胆碱再进一步通过不同的HPLC系统分离二亚油酰磷脂酰胆碱,并通过标准样品和质谱进行二亚油酰磷脂酰胆碱的定性.定量方法通过标准样品外标法定量,由方法的回收率和精密度试验可知,该方法已用来测定一般磷脂样品和磷脂酰胆碱样品中的二亚油酰磷脂酰胆碱含量.

关 键 词:二亚油酰磷脂酰胆碱  磷脂酰胆碱  高效液相色谱
文章编号:1000-0720(2007)03-011-04
修稿时间:2006-07-312006-09-03

A analysis of dilinoleoylphosphatidylcholine
CAO Dong. A analysis of dilinoleoylphosphatidylcholine[J]. Chinese Journal of Analysis Laboratory, 2007, 26(3): 11-14
Authors:CAO Dong
Affiliation:Food School, Yangtze University, Wuxi 214122
Abstract:The analytical method of dilinoleoylphosphatidyleholine was studied in this paper. Phosphatidyleholine was separated from phospholipid by HPLC, and then the dilinoleoylphosphatidyleholine was separated from the obtained phosphatidyleholine by another HPLC system. Qualitative analysis of dilinoleoylphosphatidyleholine was completed by MS with the aid of standard sample. And quantitative analysis of dilinoleoylphosphatidyleholine was completed with the extrnal standard method. The method was applied to the analysis of dilinoleoylphosphatidyleholine in phospholipid and phosphatidyleholine samples. It is accurate, reliable and convenient by precision and recovery tests.
Keywords:Dilinoleoylphosphatidyleholine   Phosphatidyleholine   HPLC
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