Optimization of enzyme catalyzed peptide synthesis in a “water - water-immiscible organic solvent” biphasic system

Optimal conditions were found, for enzymatic synthesis of the dipeptide, N-acetyl-L-tryptophanyl-L-leucine amide in the biphasic system water - ethyl acetate. The synthesis was carried out using both free and. immobilized α-chymotrypsin. Optimization was performed, by such parameters as the “organic phase/aqueous phase” volume ratio, the pH of aqueous phase, and the concentration of starting reactants. Under most favourable condition the dipeptide was synthesized. on the preparative scale in ca. 100% yield. As a result of immobilization (adsorption on the Sorsilen terephtalate support) the enzyme practically did not inactivate and may be used repeatedly.