Dependence of the methylene selectivity on the composition of hydro-organic eluents for reversed-phase liquid chromatographic systems with alkyl bonded phases |
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Authors: | R K Gilpin M Jaroniec S Lin |
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Institution: | (1) Department of Chemistry, Kent State University, 44242 Kent, Ohio, USA;(2) Present address: Chemistry Faculty, M. Curie-Sklodowska University, 20031 Lublin, Poland |
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Abstract: | Summary A theoretical treatment is presented which considers differences between the composition of the mobile phase and solvents
that are incorporated into the bonded phase via preferential sorption. Equations are derived and used to analyze retention
data for various homologs chromatographed under reversed-phase conditions using alkyl bonded phases and combinations of water-methanol,
water-acetonitrile and watertetrahydrofuran as mobile phases. In the case of water-methanol the surface phase and bulk mobile
phase compositions are similar. However, significant differences in composition between the two phases are observed when binary
combinations of water-acetonitrile and water-tetrahydrofuran are used as the cluents. |
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Keywords: | Column liquid chromatography Alkyl bonded phases Hydro-organic mobile phases Methylene selectivity Stationary phase composition |
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