Synthetic peptides with selective affinity for apoptotic cells |
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Authors: | DiVittorio Kristy M Johnson James R Johansson Emma Reynolds Aaron J Jolliffe Katrina A Smith Bradley D |
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Affiliation: | Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA. |
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Abstract: | The appearance of anionic phosphatidylserine (PS) in the outer monolayer of the plasma membrane is a universal indicator of the early/intermediate stages of cell apoptosis. The most common method of detecting PS on a cell surface is to use the protein annexin V; however, in certain applications there is a need for alternative reagents. Recent research indicates that rationally designed zinc 2,2'-dipicolylamine (Zn2+-DPA) coordination complexes can mimic the apoptosis sensing function of annexin V. Here, a series of fluorescently-labelled, tri- and pentapeptides with side chains containing Zn2+-DPA are prepared and shown to selectively bind to anionic vesicle membranes. Fluorescein-labelled versions of the peptides are used to detect apoptotic cells by fluorescence microscopy and flow cytometry. |
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