反相高效液相色谱法测定3种中成药中的葛根素

 建立了测定小儿清感灵片、步长新脑心通胶囊和感冒清热颗粒 3种中成药中葛根素含量的反相高效液相色谱方法。采用APEXODS色谱柱 ,以醋酸铵缓冲液 (10 0mmol/L ,pH 5 0 ) 甲醇 (体积比为 75∶2 5 )的混合溶液为流动相 ,检测波长为 2 5 0nm ,流速为 0 8mL/min。葛根素在 2mg/L～ 2 0mg/L时其色谱峰面积与质量浓度的线性关系良好 (r =0 9999) ;上述 3种中成药中的葛根素含量分别为 3 48mg/g ,1 0 8mg/ g及 1 5 2mg/ g(蔗糖型 ) ;其加样回收率分别为 99 0 % ,93 4%和 97 5 %。该法简便、快速、专属性强 ,可以作为多种中药制剂中葛根素含量的测定方法。

Determination of Puerarin in Three Traditional Chinese Patent Medicines by High Performance Liquid Chromatography

A simple and accurate reversed-phase high performance liquid chromatographic method to determine puerarin in three traditional Chinese patent medicines, namely "Xiao'er qingganling" tablet, "Buchang xinnaoxintong" capsulae and "Ganmao qingre" granule is described. "Xiao'er qingganling" tablet and "Buchang xinnaoxintong" capsulae were extracted with methanol in an ultrasonic bath and "Ganmao qingre" granule was extracted with water and ethanol. After being centrifuged and filtered, the samples were separated on an ODS column with a mobile phase of 100 mmol/L ammonium acetate buffer (pH 5.0)-methanol(75:25, volume ratio). The flow rate was 0.8 mL/min, and the detection wavelength was 250 nm. The calibration curve was linear in the range of 2 mg/L-20 mg/L puerarin (r = 0.9999). The average recoveries were 99.0%, 93.4% and 97.5% for "Xiao'er qingganling" tablet, "Buchang xinnaoxintong" capsulae, "Ganmao qingre" granule respectively. The average contents of puerarin in the medicines were 3.48 mg/g, 1.08 mg/g and 1.52 mg/g (sucrose), and the relative standard deviations were 1.9%, 1.4% and 1.4% respectively.