Assay of trimethoprim,sulfamethoxazole and its N4-acetyl metabolite in biological fluids by high-pressure liquid chromatography |
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| Authors: | V. Ascalone |
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| Abstract: | A reversed-phase HPLC assay method was developed to determine levels of the drugs trimethoprim, sulfamethoxazole and the N4-acetyl metabolite of the latter in human blood plasma and urine. The organic substances are extracted with ethyl acetate by a single extraction at pH 6.8. The method is rapid, accurate, sensitive and precise and well suited to routine determination of these drugs on patients during clinical pharmacokinetic investigations. |
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| Keywords: | Column liquid chromatography, HPLC Quantitative analysis in human plasma extracts TMP, SMZ, SMZ-N4 acetyl derivatives in μ g/ml range Recovery data |
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