| Abstract: | Analytical procedures have been investigated for the separation, detection, identification and quantitation of some metabolites of N-benzyl-4-substituted anilines. Techniques based on gas-liquid chromatography were investigated and found to be unsatisfactory. By the use of reversed-phase high-performance liquid chromatography with gradient and ion-pairing techniques, methods were devised for the simultaneous analyses of a variety of metabolites. The method involves minimum sample work-up (acetonitrile precipitation) and allows easy and prompt analysis in biological media avoiding undue decomposition of unstable metabolites. |
