| 固定HCG抗体膜的跨膜电位 |
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| 引用本文： | 吕鸣祥,方道斌,张杰,陈昆明.固定HCG抗体膜的跨膜电位[J].物理化学学报,1992,8(4):523-526. |
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| 作者姓名： | 吕鸣祥 方道斌 张杰 陈昆明 |
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| 作者单位： | Department of Applied Chemistry, Tianjin University, Tianjian 300072; Tianjin Medical College |
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| 基金项目： | 中科院长春应化所电分析开放研究室资助课题 |
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| 摘 要： | 高分子膜作为一项新兴技术,在很多领域得到日益广泛的应用.近十几年,随着生物工程和生物传感器的迅速发展,高分子生物功能膜的研究倍受重视.高分子生物功能膜是采用固定化技术,将具有分子识别功能的材料(如酶、抗原、抗体等)固定在高分子膜上而制得的.在固定化膜表面发生的生物化学反应,可以引起膜的荷电状态的变化,从而导致跨膜电位的变化。有关固定化膜的报导较多,但主要限于固定化的方法及其应用方面的研究,而有关高分
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| 关 键 词： | 人绒毛膜促性腺素(HCG) 跨膜电位 兔疫化学反应 |
| 收稿时间： | 1991-02-27 |
| 修稿时间： | 1991-07-23 |
THE TRANSMEMBRANE POTENTIAL OP HCG ANTIBODY-BINDING MEMBRANE |
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| Lu Mingxiang,Fang Daobin,Zhang Jie,Chen Kunming.THE TRANSMEMBRANE POTENTIAL OP HCG ANTIBODY-BINDING MEMBRANE[J].Acta Physico-Chimica Sinica,1992,8(4):523-526. |
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| Authors: | Lu Mingxiang Fang Daobin Zhang Jie Chen Kunming |
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| Institution: | Department of Applied Chemistry, Tianjin University, Tianjian 300072; Tianjin Medical College |
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| Abstract: | In this paper the antibody of HCG was immobilzed on the cellulose acetate membrane. The membrane potentials varied with the electrolyte concentrations of the two aqueous phases. The charge density of the membrane phase was lowered as the immunochemical reaction of HCG with HCG antibody proceeded on the membrane surface. When c_1/c_2=8.0, the antibody-bound membrane potentials shifted to posi-tive values with the increase of HCG concentrations. The membrane immunosensor for the measurement HCG in the solution was constructed according to the change of the membrane potential. A linearity between Δφ and lg HCG can be obtained in the concentration range of 50—2500 mlU·ml~(-1) HCG. |
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| Keywords: | Human chorionic gonadotropin(HCG) Transmembrane potential Immunochemical reaction |
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