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| 高效液相色谱-质谱法快速鉴定复方毛冬青冲剂中三萜皂苷活性成分 | |
| 引用本文： | 杨运云,郭鹏然,陈怡禄,邓六勤,钟鸣,吴庆晖.高效液相色谱-质谱法快速鉴定复方毛冬青冲剂中三萜皂苷活性成分[J].分析化学,2009,37(10). |
| 作者姓名： | 杨运云 郭鹏然 陈怡禄 邓六勤 钟鸣 吴庆晖 |
| 作者单位： | 1. 中国广州分析测试中心,广东省化学危害应急检测技术重点实验室,广州,510070 2. 广州市儿童医院,广州,510120 3. 广州汉方现代中药研究开发有限公司,广州,510240 |
| 摘 要： | 建立了高效液相色谱-质谱法(HPLC-MS)快速鉴定复方毛冬青冲剂中三萜皂苷活性成分的方法.以甲醇为萃取剂超声萃取复方毛冬青冲剂30 min.采用高效液相色谱-离子阱质谱(HPLC-IT-MS)和高效液相色谱-飞行时间质谱(HPLC-TOF-MS)对萃取液进行分析,选用Agilent Zorbax Eclipse XDB C18色谱柱(250 mm×4.6 mm, 5 μm),以水(含0.1 %甲酸)-乙腈为流动相进行梯度洗脱,柱后流出液采用电喷雾离子阱质谱(ESI-IT-MS)和电喷雾飞行时间质谱(ESI-TOF-MS)的正、负离子模式进行检测.检测结果经离子阱一级质谱(IT-MS1)、离子阱二级质谱(IT-MS2)和分析时间质谱(TOF-MS)信息分析,并与相关文献报道进行比较,鉴定出1种三萜酸和8种三萜皂苷成分,并推测了其它3种可能的三萜皂苷化学成分,通过对照品对比分析,三萜酸确证为Ilexgenin A,其中一种三萜皂苷确证为Ilexsaponin A1.本方法无需对照品即可快速有效地鉴定出复方毛冬青冲剂中的三萜皂苷活性成分,为建立冲剂的质量标准提供了依据. |
| 关 键 词： | 复方毛冬青冲剂 三萜皂苷 高效液相色谱-质谱 |
Rapid Identification of Triterpene and Saponin in Compound Radix Ilicis Pubescentis Medicinal Granules by High Performance Liquid Chromatography-Mass Spectrometry |
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| YANG Yun-Yun,GUO Peng-Ran,CHEN Yi-Lu,DENG Liu-Qin,ZHONG Ming,WU Qing-Hui.Rapid Identification of Triterpene and Saponin in Compound Radix Ilicis Pubescentis Medicinal Granules by High Performance Liquid Chromatography-Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2009,37(10). | |
| Authors: | YANG Yun-Yun GUO Peng-Ran CHEN Yi-Lu DENG Liu-Qin ZHONG Ming WU Qing-Hui |
| Institution: | YANG Yun-Yun1,GUO Peng-Ran1,CHEN Yi-Lu2,DENG Liu-Qin2,ZHONG Ming3,WU Qing-Hui11(Guangdong Key Laboratory of Chemical Emergency Test,China National Analytical Center,Guangzhou 510070)2(Guangzhou Children's Hospital,Guangzhou 510120)3(Guangzhou Hanfang Natural Medicine Research & Development Co.Ltd.,Guangzhou 510240) |
| Abstract: | A method was developed for the rapid identification of triterpene and saponin in compound Radix ilicis pubescentis medicinal granules by high performance liquid chromatography-mass spectrometry(HPLC-MS).The sample was extracted with methanol by ultrasonic wave extraction for 30 min.The extract was analyzed by high performance liquid chromatography-ion trap-mass spectrometry(HPLC-IT-MS) and high performance liquid chromatography-time of fight-mass spectrometry(HPLC-TOF-MS).An Agilent Zorbax Eclipse XDB C18 c... |
| Keywords: | Compound Radix ilicis pubescentis medicinal granules triterpene and saponin high performance liquid chromatography-mass spectrometry |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |