Proteomics Dissection of Cold Responsive Proteins Based on PEG Fractionation in Arabidopsis |
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Authors: | LI Shanyu LIU Xiangguo WANG Shang HAO Dongyun XI Jinghui |
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Institution: | 1. College of Plant Science, Jilin University, Changchun 130062, P. R. China;
2. The First Hospital of Jilin University, Changchun 130021, P. R. China;
3. Biotechnology Research Centre, Jilin Academy of Agricultural Sciences, Changchun 130124, P. R. China |
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Abstract: | Proteome profiling was performed on Arabidopsis plant exposed to cold stress at 4 ℃ for 24 h in an attempt to explore the mechanisms of plant climate adaptation. The polyethylene glycol(PEG) fractionation protocol developed in this lab was used to identify as many differentially expressed low-abundance proteins as possible. In comparison with those of the biological controls, 67 protein spots with at least two-fold difference in expression were identified for the plant exposed to cold temperatures; and from these spots, 50 proteins were successfully identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS). Bioinformatics studies on these proteins show that 57.8% of these proteins were localized in the chloroplast. Of these proteins, 8 ones have functions in photosynthesis, including glycine hydroxymethyltransferase, Rubisco large subunit, Rubisco activase, PSBO2, fructose-1,6-bisphosphate aldolase, NADP-dependent malate dehydrogenase, sedoheptulose bisphosphatase and photosystem Ⅱ reaction center PsbP family protein, suggesting that photosynthesis is greatly affected by cold stress. The identified proteins were validated by quantitative real-time polymerase chain reaction(qPCR). Taken together, our results suggest that the chloroplast might also act as a cold stress sensor and that photosynthesis-related proteins may play important roles in cold acclimation for Arabidopsis. |
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Keywords: | Arabidopsis thaliana Cold stress Proteome Protein fractionation |
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