Identification and characterization of Se-methyl selenomethionine in Brassica juncea roots |
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Authors: | Grant Tyre D Montes-Bayón María LeDuc Danika Fricke Michael W Terry Norman Caruso Joseph A |
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Affiliation: | Department of Chemistry, University of Cincinnati, PO Box 0172, Cincinnati, OH 45221-0172, USA. |
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Abstract: | The present work shows the identification and characterization of Se-methyl selenomethionine (SeMMet) as an important Se species in Brassica juncea roots when grown in the presence of Se-methionine (SeMet) as the Se source. SeMMet was isolated by liquid chromatography employing two different liquid chromatographic mechanisms: reversed-phase ion-pairing using heptafluorobutyric acid as counter ion and cation exchange using a pyridinium formate gradient (pH 3). Inductively coupled plasma mass spectrometry was used for the detection of Se. SeMMet was characterized by electrospray quadrupole time-of-flight MS in both a synthesized standard and in the roots extract using collision-induced dissociation of the selected ion. Preliminary evidence suggests that Brassica juncea may also produce dimethylselenonium propionate, although to a much lesser extent. |
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