Protective effect of transgenic expression of porcine heat shock protein 70 on hypothalamic ischemic and oxidative damage in a mouse model of heatstroke |
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Authors: | Zhih-Cherng Chen Wen-Shian Wu Mao-Tsun Lin Chuan-Chih Hsu |
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Institution: | (1) Section of General Medicine, Chi Mei Medical Center, Tainan, Taiwan, Republic of China;(2) Chung Hwa University of Medical Technology, Tainan, Taiwan, Republic of China;(3) Department of Cardiology, Chi Mei Medical Center, Tainan, Taiwan, Republic of China;(4) Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan, Republic of China;(5) Department of Surgery, Chi Mei Medical Center, Tainan, Taiwan, Republic of China |
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Abstract: | Background Transgenic mice have been used to examine the role of heat shock protein (HSP)72 in experimental heatstroke. Transgenic mice
that were heterozygous for a porcine HSP70β gene (+] HSP72) and transgene-negative littermate controls (-] HSP72), under
pentobarbital sodium anesthesia, were subjected to heat stress to induce heatstroke. It was found that the overexpression
of HSP72 in multiple organs improved survival during heatstroke by reducing hypotension and cerebral ischemia and damage in
mice. Herein we attempted to further assess the effect of heat exposure on thermoregulatory function, hypothalamic integration,
and survival in unrestrained, unanesthetized +]HSP72 and compare with those of -]HSP72. In this research with the transgenic
mice, we first conducted several biochemical, physiologic and histological determinations and then investigated the beneficial
effects of HSP72 overexpression on the identified hypothalamic deficits, thermoregulatory dysfunction, and mortality during
heatstroke. |
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