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Enzyme-mediated biodegradation of heat treated commercial polyethylene by Staphylococcal species |
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| Authors: | Sumana Chatterjee Bappaditya Roy Rajat Banerjee |
| Institution: | a Department of Chemistry, Basanti Devi College, 147 B, Rashbehari Avenue, Kolkata 700029, India b Dr. B. C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India c Department of Polymer Science and Technology, University of Calcutta, 92 A.P.C Road, Kolkata 700009, India |
| Abstract: | Extruded low-density polyethylene (LDPE) films commonly available in the market as 20-micron thick carrier bags were autoclaved, overlaid on nutrient agar plates and inoculated with BP/SU1 strain of Staphylococcus epidermis. The nutrient agar plate showed growth of the organism within two to three days. The polymer film supporting the growth of the organism showed pore formation as recorded by SEM analysis. The growth of BP/SU1 is supported by the presence of shredded LDPE as its only carbon source in inorganic salt minimal nutrient medium. The organism survives even after three months of inoculation and this is accompanied by gradual breakdown of the size of the shredded plastic as seen by light scattering. The cell-free supernatant of the organism, grown with the help of shredded plastic shows the presence of the over expressed proteins with approximate molecular weight of about 55 kDa and 35 kDa, through SDS-PAGE analysis. |
| Keywords: | LDPE Staphylococcus epidermis BP/SU1 Biodegradation SEM FT-IR |
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