Evaluation of jatropha oil to produce poly(3-hydroxybutyrate) by Cupriavidus necator H16 |
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Authors: | Ko-Sin Ng Lay-Koon Goh Kumar Sudesh |
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Affiliation: | a Ecobiomaterial Research Laboratory, School of Biological Science, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia b Department of Biotechnology, Lady Doak College, Madurai 625002, Tamil Nadu, India |
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Abstract: | Jatropha oil, a non-edible vegetable oil, may be an alternative substrate to food-grade oils for bioplastic production. Jatropha oil contains 93.9% palmitic acids, oleic acids and linoleic acids. High P(3HB) accumulation of 87 wt% from 13.1 g/L of cell dry weight (CDW) was obtained by Cupriavidus necator H16 when 12.5 g/L of jatropha oil and 0.54 g/L of urea were used. Lipase activity increased in the initial stages of P(3HB) production, when 1 g/L of jatropha oil was added to the preculture medium. Addition of oil in preculture did not affect final CDW or P(3HB) accumulation. P(3HB) production in a 10 L lab-scale fermenter gave a yield of 0.78 g P(3HB) per g jatropha oil used after 48 h. For the first time, this study proved that jatropha oil is a feasible and excellent carbon source for P(3HB) biosynthesis by C. necator H16 with potential for large-scale production. The toxins in jatropha oil did not affect the P(3HB) biosynthesis. |
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Keywords: | Jatropha oil Ratanjyot oil Physic nut Poly(3-hydroxybutyrate) Bioplastics |
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