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The effect of sterilization methods on the thermo-gelation properties of xyloglucan hydrogels |
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| Authors: | Amanda K Andriola Silva Brun-Graeppi Cyrille Richard Michel Bessodes Daniel Scherman Tetsuharu Narita Otto-Wilhelm Merten |
| Institution: | a Université d'Évry Val d'Essonne, École doctorale des Génomes Aux Organismes, Boulevard François Mitterrand, 91025 Evry Cedex, France b Genethon, 1 bis rue de l'Internationale, BP 60, Evry Cedex 91002, France c Unité de Pharmacologie Chimique et Génétique, CNRS, UMR 8151, Paris, F-75270 Cedex, France d Inserm, U 640, Paris, F-75270 Cedex, France e Université Paris Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, F-75270 Cedex, France f ENSCP, Paris, F-75231 Cedex, France g Physico-chimie des Polymères et des Milieux Dispersés, UMR 7615, UPMC-CNRS-ESPCI, 10 rue Vauquelin, 75231 Paris Cedex 05, France |
| Abstract: | In this study, the influence of different sterilization methods on the thermo-gelation and structural properties of xyloglucan hydrogels was investigated. Xyloglucan samples were treated by either 70% ethanol, 70% isopropanol, γ-irradiation (10 kGy) at room temperature, γ-irradiation (10 kGy) in dry ice or autoclaving. These samples were tested for sterility by incubation with sterile Lysogeny Broth (LB) at room temperature, 30 °C and 37 °C for 30 days. According to the results obtained, xyloglucan hydrogels were only effectively sterilized by autoclaving or by γ-irradiation either at room temperature or in dry ice. These samples were analyzed by rheology measurements and dynamic and static light scattering analysis. Gamma-irradiation at room temperature markedly changed the polymer structure, preventing thermo-gelation. Only autoclaving and γ-irradiation in dry ice preserved the rheological properties of the polymer. The sol-gel transition as a function of the temperature was similar for these samples and the control sample. |
| Keywords: | Sterilization Thermo-gelation Xyloglucan Degradation |
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