Neural differentiation potential of human bone marrow-derived mesenchymal stromal cells: misleading marker gene expression |
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| Authors: | Katrin Montzka Nina Lassonczyk Beate Tsch?ke Sabine Neuss Tobias Führmann Rachelle Franzen Ralf Smeets Gary A Brook and Michael W?ltje |
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| Institution: | (1) Department of Neurology, RWTH Aachen University, Aachen, Germany;(2) Institute for Neuropathology, RWTH Aachen University, Aachen, Germany;(3) Interdisciplinary Center for Clinical Research (IZKF) 'BIOMAT.', RWTH Aachen University, Aachen, Germany;(4) Department of Biomedical Engineering, RWTH Aachen University, Aachen, Germany;(5) Institute of Pathology, RWTH Aachen University, Aachen, Germany;(6) Research Center for Cellular and Molecular Neurobiology, University of Li?ge, Li?ge, Belgium;(7) Clinics for Oral and Maxillofacial Surgery, RWTH Aachen University, Aachen, Germany;(8) Roche Diagnostics GmbH, Penzberg, Germany |
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| Abstract: | Background In contrast to pluripotent embryonic stem cells, adult stem cells have been considered to be multipotent, being somewhat more
restricted in their differentiation capacity and only giving rise to cell types related to their tissue of origin. Several
studies, however, have reported that bone marrow-derived mesenchymal stromal cells (MSCs) are capable of transdifferentiating
to neural cell types, effectively crossing normal lineage restriction boundaries. Such reports have been based on the detection
of neural-related proteins by the differentiated MSCs. In order to assess the potential of human adult MSCs to undergo true
differentiation to a neural lineage and to determine the degree of homogeneity between donor samples, we have used RT-PCR
and immunocytochemistry to investigate the basal expression of a range of neural related mRNAs and proteins in populations
of non-differentiated MSCs obtained from 4 donors. |
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