MALDI-MS imaging of lipids in ex vivo human skin |
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Authors: | Philippa J Hart Simona Francese Emmanuelle Claude M Nicola Woodroofe Malcolm R Clench |
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Institution: | (1) Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield, S1 1WB, UK;(2) Waters Corporation, Atlas Business Park, Simonsway, Manchester, M22 5PP, UK; |
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Abstract: | Lipidomics is a rapidly expanding area of scientific research and there are a number of analytical techniques that are employed
to facilitate investigations. One such technique is matrix-assisted laser desorption ionisation (MALDI) mass spectrometry
(MS). Previous MALDI-MS studies involving lipidomic investigation have included the analysis of a number of different ex vivo
tissues, most of which were obtained from animal models, with only a few being of human origin. In this study, we describe
the use of MALDI-MS, MS/MS and MS imaging methods for analysing lipids within cross-sections of ex vivo human skin. It has
been possible to tentatively identify lipid species via accurate mass measurement MALDI-MS and also to confirm the identity
of a number of these species via MALDI-MS/MS, in experiments carried out directly on tissue. The main lipid species detected
include glycerophospholipids and sphingolipids. MALDI images have been generated at a spatial resolution of 150 and 30 μm,
using a MALDI quadrupole time-of-flight Q-Star Pulsar-i
TM (Applied Biosystems/MDS Sciex, Concord, ON, Canada) and a MALDI high-definition MS (HDMS) SYNAPT G2-HDMSTM system (Waters, Manchester, UK), respectively. These images show the normal distribution of lipids within human skin, which
will provide the basis for assessing alterations in lipid profiles linked to specific skin conditions e.g. sensitisation,
in future investigations. |
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