He-Ne激光对钝顶螺旋藻的诱变效应

利用He-Ne激光(波长632 nm，功率10 mW)诱变钝顶螺旋藻IS（Spirulina platensis IS），经过一定时间的辐照，选取生长较快的藻种测量其β-胡萝卜素、蛋白质及多糖含量，进一步筛选出生长快、高产胡萝卜素或高产蛋白质的藻种.实验结果表明：与出发藻种IS相比，经He-Ne激光15min和25 min照射后的藻种MS-1、MS-2和 MS-3，藻丝形态发生变化，藻丝变短、螺旋变紧密，生长速度明显加快，其中 MS-1的β-胡萝卜素含量增幅为18.1%，MS-3的蛋白质和多糖含量均有较大增加.此外通过对出发藻种和诱变藻种的叶绿素a和胡萝卜素的紫外吸收光谱进行比较，发现诱变藻种与出发藻种相比最大吸收峰值略有变化，从而说明He-Ne激光对于钝顶螺旋藻的诱变效应.

Studies on the Mutagenesis of Spirulina Platensis Using He-Ne Laser

Spirulina platensis was mutated by He-Ne Laser (wave length 632 nm,power 10 mW),irradiated for some time,some strains which grow faster than others were chosen to measure the content of β-carotene,protein and polysaccharide,then the strains with high production,high content of β-carotene and protein ext were selected.The result shows that,spirulina platensis sp MS-1,MS-2 and MS-3 which were irradiated by He-Ne laser for 15 min and 25 min,compared with original strain IS,its morphologh parameter changed,morphologh parameter shorted,helix tighted and grow speed was increased obviously.The content of β-carotene of MS-1 was increased by 18.1%,the content of protein and polysaccharide of MS-3 were also increased.In addition,the peak value of original strain was changed compared with the mutant strains by the ultraviolet spectrum of chlorophyll and carotenoid.It showed the mutagenesis domino effect of spirulina platensis by He-Ne Laser.