高效液相色谱法测定喘静片的3种主要成分

 采用高效液相色谱法同时测定喘静片中的3种主要成分：茶碱、异戊巴比妥和盐酸甲基麻黄碱。以ODS作固定相，通过均匀设计方法选择的最佳流动相为V（0.015 mol/L磷酸二氢钠缓冲液，含体积分数为0.3%的三乙胺，pH 4.9 )∶V（甲醇）=35∶65，检测波长为215 nm，用程序调节检测器的灵敏度，以咖啡因作内标物。茶碱、异戊巴比妥、盐酸甲基麻黄碱的回收率分别为99.7%～102.6%，98.5%～100.2%，98.0%～102.7%，日间RSD分别为0.23%～1.2%，0.35%～2.5%，0.33%～1.6%。该方法简便、快速、准确。

Determination of Three Principal Components in Chuanjing Tablets by Using High Performance Liquid Chromatography

Uniform design method was employed to optimize the mobile phase of HPLC in order to determine simultaneously three principal components, theophylline, amobarbital and methylephedrine hydrochloride, in Chuanjing tablet, a compound preparation for asthma and cough. The stationary phase was ODS and the optimal mobile phase composition was V (0.015 mol/L phosphate buffer containing 0.3% triethylamine, pH 4.9): V (methanol) = 35:65. The detection was performed at 215 nm and the sensitivity was time programmed for simultaneous determination of minor and major components. Caffeine was selected as the internal standard. A baseline separation was achieved within 10 min. The linear ranges for theophylline, amobarbital and methylephedrine hydrochloride were 0.03 g/L-0.20 g/L, 7.5 mg/L-50.0 mg/L and 7.5 mg/L-50.0 mg/L, recoveries 99.7%-102.6%, 98.5%-100.2% and 98.0%-102.7%, inter-day RSDs 0.23%-1.2%, 0.35%-2.5%, 0.33%-1.6% respectively. This HPLC method is rapid and accurate, and suitable for the quality control of the preparation.