Cellular Effects of Cyclodextrins: Studies on HeLa Cells |
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Authors: | Á gnes Rusznyá k,Mercé desz Palicskó ,Milo Malanga,É va Fenyvesi,Lajos Szente,Judit Vá radi,Ildikó Bá cskay,Mikló s Vecsernyé s,Katalin Szá szné Ré ti-Nagy,Gá bor Vasvá ri,Á dá m Haimhoffer,Ferenc Fenyvesi |
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Abstract: | Cyclodextrins are high molecular weight, hydrophilic, cyclic, non-reducing oligosaccharides, applied as excipients for the improvement of the solubility and permeability of insoluble active pharmaceutical ingredients. On the other hand, beta-cyclodextrins are used as cholesterol sequestering agents in life sciences. Recently, we demonstrated the cellular internalization and intracellular effects of cyclodextrins on Caco-2 cells. In this study, we aimed to further investigate the endocytosis of (2-hydroxylpropyl)-beta-(HPBCD) and random methylated-beta-cyclodextrin (RAMEB) to test their cytotoxicity, NF-kappa B pathway induction, autophagy, and lysosome formation on HeLa cells. These derivatives were able to enter the cells; however, major differences were revealed in the inhibition of their endocytosis compared to Caco-2 cells. NF-kappa B p65 translocation was not detected in the cell nuclei after HPBCD or RAMEB pre-treatment and cyclodextrin treatment did not enhance the formation of autophagosomes. These cyclodextrin derivates were partially localized in lysosomes after internalization. |
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Keywords: | HPBCD, RAMEB, endocytosis, lysosomes, autophagy, NF-κ B, HeLa |
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