槲皮素在聚L-络氨酸修饰电极上的电化学行为及测定

采用电聚合方法制备了聚L-络氨酸修饰电极。利用循环伏安法(CV)探究了pH值、扫描速率对槲皮素电化学行为的影响。用差分脉冲伏安法(DPV)对槲皮素进行测定。结果表明:聚L-络氨酸修饰电极在pH值为6.0的磷酸盐缓冲溶液中对槲皮素表现出良好的电催化能力。在6.21×10~(-5)～6.9×10~(-4) mol/L范围内槲皮素的浓度与相应的检测信号呈现出良好的线性关系,线性方程为:I(10~(-6) A)=-1.034 8-0.099 39c(10~(-4) mol/L),线性相关系数R=-0.987 87,检出限为2.07×10~(-5) mol/L(S/N=3)。电化学分析方法简易快捷、重现性和稳定性高。

Electrochemical Behaviors and Determination of Quercetin at Poly (L-Lysine) Modified Electrode

A poly (L-Lysine) modified electrode was prepared by electro-polymerization technology. The electrochemical behavior on pH and scan rate was investigated by cyclic voltammetry (CV). The contents of quercetin were determined using differential pulse voltammetry (DPV). The results indicated that the modified electrode showed a good electric catalytic ability for quercetin in phosphate buffer solution with pH=6.0. Linear relationship between the oxidation peak current and the concentrations of quercetin was obtained in the range of 6.21×10-5~6.9 ×10-5 mol/L. The linear equation was I (10-6A) =1.034 8-0.099 39 c(10-4 mol/L) with correlation coefficient (R) of-0.98787. The detection limit (S/N=3) was 2.07×10-5 mol/L. The method has fast analytical speed, good accuracy, high reproducibility and stability.