Electrophoretic behavior of peptides in capillary electrophoresis influence of ionic strength and pH in aqueous-organic media. |
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| Authors: | V Sanz-Nebot F Benavente I Toro J Barbosa |
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| Institution: | Department of Analytical Chemistry, University of Barcelona, Spain. vsanz@apolo.qui.ub.es |
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| Abstract: | Through correct pH, pKa and activity coefficients values, a model describing the effect of pH on electrophoretic mobility of substances has been applied to a series of peptides in water and in acetonitrile-water mixtures. The derived equations permit prediction of the optimum pH for the electrophoretic separation from only a few experimental values and they also permit determination of pKa values of analytes in the aqueous-organic media employed. Furthermore, the electrophoretic resolution between pairs of substances can be predicted, in order to evaluate electrophoretic separations of the studied peptides. |
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