Use of linear solvation energy relationships for characterization of hypercrosslinked polystyrene stationary phases

Linear solvation energy relationships (LSER model) was tested for the characterization of hypercrosslinked polystyrene (HCPS) stationary phases for high-performance liquid chromatography (HPLC). Analysis of LSER coefficients showed that hydrophobic and electrostatic interactions are the major contributors to retention on HCPS. Fluorine atoms in HCPS increase the fractions of both hydrophobic and electrostatic interactions in the retention. The utility of fluorinated HCPS in the separation of di-n-phthalate mixtures by reversed-phase liquid chromatography was demonstrated.