Molecular engineering of NLO-phores for new NLO microscopies |
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Affiliation: | Synthèse et electrosynthèse organiques (CNRS UMR 6510), Institut de chimie, Université de Rennes 1, Campus scientifique de Beaulieu, bâtiment 10A, 35042 Rennes, France |
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Abstract: | Novel microscopies based on nonlinear optical (NLO) phenomena such as two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) have gained overwhelming popularity in the biology community owing to the many advantages they provide in biological imaging. Examples of molecular engineering approaches toward NLO-probes specifically designed for SHG and/or TPEF imaging of lipid membranes and biological cells are given here, providing an illustration of their intriguing potential in the area of real-time, non-damaging imaging of biological structures. Optimized NLO-markers open new routes for improved monitoring and better understanding of fundamental dynamic processes. To cite this article: M. Blanchard-Desce, C. R. Physique 3 (2002) 439–448. |
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