Dynamic capillary coatings for electroosmotic flow control in capillary electrophoresis |
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| Institution: | 1. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China;2. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China;1. KU Leuven—University of Leuven, Pharmaceutical Analysis, Department of Pharmaceutical and Pharmacological Sciences, O&N2, PB 923, Herestraat 49, 3000 Leuven, Belgium;2. Analytical Pharmaceutical Chemistry, Uppsala University, 751 23 Uppsala, Husargatan 3, Sweden;3. Kantisto BV, Callenburglaan 22, 3742 MV Baarn, The Netherlands;1. Department of Mathematics, Jadavpur University, Kolkata - 700032, India;2. Department of Mathematics, Yogoda Satsanga Palpara Mahavidyalaya, Purba Medinipur-721457, India;1. Institute of Analytical Chemistry of the Czech Academy of Sciences, Veve?í 97, CZ-60200, Brno, Czech Republic;2. Department of Chemistry, University of Basel, Klingelbergstrasse 80, CH-4056, Basel, Switzerland |
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| Abstract: | Control of the electroosmotic flow (EOF) is critical for achieving optimal separations by capillary electrophoresis. For instance, manipulation of the EOF can yield either high resolution separations or rapid analyses. Dynamic capillary coatings are a simple and cost-effective approach to altering the EOF. The normal EOF can be slowed using buffer additives such as Mg2+ and hexamethonium which ion exchange onto the surface silanols to lower the effective wall charge. Alternatively, cationic polyelectrolytes or cationic surfactants can be used to establish a cationic coating on the capillary wall, which results in a reversed EOF. Practical considerations such as pH stability and reproducibility obtainable with an EOF modifier will be discussed. |
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